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RT-PCR. Reverse transcription polymerase chain reaction ( RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [ 1] It is primarily used to measure the amount of a specific RNA.
Master mix (PCR) A master mix is a mixture containing precursors and enzymes used as an ingredient in polymerase chain reaction techniques in molecular biology. Such mixtures contain a mixture dNTPs (required as a substrate for the building of new DNA strands), MgCl 2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer ...
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
A small number of Netflix users are receiving a message asking them to confirm they live with the account owner by entering details from a text message or email sent to the owner. Viewers can ...
The return to classrooms in the fall usually means kids bring back germs along with their homework. Dr. Leana Wen has advice to make the transition easier.
A real-time polymerase chain reaction ( real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.
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