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ELISA. The enzyme-linked immunosorbent assay ( ELISA) ( / ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using ...
As a result, the DAS-ELISA principle is commonly employed in ELISA's for the detection of plant pathogens in plant sap without prior purification of the pathogen. The ELISA is considered to be a safe, inexpensive and rapid method for detection of plant viruses.
Potato leafroll virus (PLRV) is a member of the genus Polerovirus and family Solemoviridae. The phloem limited positive sense RNA virus [1] infects potatoes and other members of the family Solanaceae. PLRV was first described by Quanjer et al. in 1916. [2] PLRV is transmitted by aphids, primarily the green peach aphid, Myzus persicae.
Immunofluorescence or immunoperoxidase. Immunofluorescence or immunoperoxidase assays are commonly used to detect whether a virus is present in a tissue sample. These tests are based on the principle that if the tissue is infected with a virus, an antibody specific to that virus will be able to bind to it.
A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants ( in vivo ), or on living cells or tissues ( in vitro ). [1] [2] A bioassay can be either quantal or quantitative, direct or indirect. [3] If the measured response is binary, the assay is quantal; if not, it is ...
This inactivation was ultimately tested by the ELISA detection method. On the contrary, lower temperatures and low heat durations have commonly failed to inactivate the virus. Another method that has been tested is cross protection. Cross protection is a type of induced resistance that is developed among plants against viruses.
An immunoassay ( IA) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other ...
black locust true mosaic virus. clover blotch virus. Peanut stunt virus ( PSV) is a plant pathogenic virus [1] in the family Bromoviridae. [2] It is a member of the genus Cucumovirus. [3] The members of this genus are positive-stranded RNA viruses with a multipartite genome (3-4 parts). The virus particles have an isometric or spherical shape.
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