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Following the reports of the first use of ELISA to detect plant viruses by Voller and Clarke (1976), Dr. Sutula introduced in 1981 the first commercial kits for the detection of common viruses and bacteria in potato. These kits provided all of the components that were necessary to perform the test in a ready-to-use format, called PathoScreen®.
ELISA. The enzyme-linked immunosorbent assay ( ELISA) ( / ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using ...
Synonyms [1] Citrus psorosis virus. Open form of the large Citrus psorosis ophiovirus particle. Negative-contrast electron micrograph (uranyl acetate); bar: 100 nm. Citrus psorosis ophiovirus is a plant pathogenic virus infecting citrus plants worldwide. [2] It is considered the most serious and detrimental virus pathogen of these trees.
Potato virus Y. Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production. PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato ...
Laboratory diagnosis of viral infections. Purpose. test for viral infection. In the diagnostic laboratory, virus infections can be confirmed by a myriad of methods. Diagnostic virology has changed rapidly due to the advent of molecular techniques and increased clinical sensitivity of serological assays. [1]
Immunoassay. An immunoassay ( IA) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may ...
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